La determine 7 représente l’observation microscopique réalisée en microscopie optique, à l’aide d’un microscope Leica® type Orthoplan, d’une coupe transversale d’un expiant cutané à J1 après traitement par P1 (P1J1), en paraffine colourées au trichrome de Masson variante de Goldner. Le grain de peau devient irrégulier surtout a

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